Ene reductase (ERED)
ES-EREDs suna haɓaka nau'ikan substrate daban-daban saboda nau'ikan substrates masu faɗi. Gabaɗaya, C=C na α, β-unsaturated mahadi tare da ƙungiyoyin shaye-shaye na electron (gami da ketone aldehyde, ƙungiyoyin nitro, carboxylic acid, esters, anhydride, lactones, imines, da sauransu) ana iya rage su cikin sauƙi ta hanyar ES-EREDs, amma haɗin biyu mara kunnawa ba.
Akwai nau'ikan samfuran enzyme na ERED guda 46 (Lambar kamar ES-ERED-101~ES-ERED-146) waɗanda SyncoZymes suka ƙirƙira.
Tsarin haɓaka kuzari:
| Enzymes | Lambar Samfura | Ƙayyadewa |
| Foda na Enzyme | ES-ERED-101~ ES-ERED-146 | saitin Ene Reductases 46, 50 MG kowanne 46 * 50mg / abu, ko wani adadi |
| Kayan tantancewa (SynKit) | ES-ERED-4600 | saitin Ene Reductases 46, 50 MG kowanne 46 * 50mg / abu, ko wani adadi |
★ Babban takamaiman substrate.
★ Ƙarfin zaɓin chiral.
★ Babban canji.
★ Rage yawan kayayyakin da aka samar.
★ Yanayin amsawa mai sauƙi.
★ Mai kyau ga muhalli.
★ Tsaro mai kyau.
➢ A al'ada, tsarin amsawar yakamata ya haɗa da substrate, maganin buffer (optimum reaction pH), coenzymes (NAD(H) ko NADP(H)), tsarin sake farfaɗo da coenzyme (misali glucose da glucose dehydrogenase) da ES-ERED.
➢ Ana iya gwada duk ES-EREDs bi da bi a cikin tsarin amsawar da ke sama ko tare da Kayan Binciken ERED (SynKit ERED).
➢ Ya kamata a yi nazarin dukkan nau'ikan ES-EREDs waɗanda suka dace da yanayin amsawar da ya dace daban-daban.
➢ Yawan sinadarin substrate ko samfurin da ke ɗauke da shi na iya hana ayyukan ES-ERED. Duk da haka, ana iya rage wannan hanawa ta hanyar ƙara sinadarin substrate a cikin rukuni.
Misali na 1(α,β-unsaturated aldehydes ko ketones)(1):
Misali na 2(α,β-unsaturated carboxylic acid da abubuwan da suka samo asali)(2):
Ajiye shi a wuri mai sanyi na tsawon shekaru 2 ƙasa da -20 ℃.
Kada a taɓa yin hulɗa da yanayi mai tsanani kamar: zafin jiki mai yawa, pH mai yawa/ƙaranci da kuma yawan sinadarin sinadarai masu narkewa.
1. Lucídio C, Fardelone J, Augusto R, da tal. J.Mol.Catal.B:Enzym., 2004, 29: 41-45.
2. Stueckler C, Hall M, Ehammer H, da sauransu. .Org.Lett, 2007, 9(26): 5409-5411.
