Ene reductase (ERED)
Es-ereds catalyze daban-daban substrate saboda fadi da fadi na substrates.Gabaɗaya, C = C na α, β-unsaturated mahadi tare da ƙungiyoyi masu shayarwa na lantarki (ciki har da ketone aldehyde, ƙungiyoyin nitro, acid carboxylic acid, esters, anhydride, lactones, imines, da sauransu) suna sauƙaƙe ta ES-EREDs, amma da unactivated biyu shaidu ba.
Akwai nau'ikan samfuran enzyme na ERED guda 46 (Lambar kamar ES-ERED-101~ES-ERED-146) wanda SyncoZymes ya haɓaka.
Na'urar catalytic:
| Enzymes | Lambar samfur | Ƙayyadaddun bayanai |
| Enzyme Foda | ES-ERED-101~ ES-ERED-146 | saitin 46 Ene Reductases, 50 MG kowane 46 abubuwa * 50mg / abu, ko wasu adadi |
| Kit ɗin Nuna (SynKit) | Saukewa: ES-ERED-4600 | saitin 46 Ene Reductases, 50 MG kowane 46 abubuwa * 50mg / abu, ko wasu adadi |
★ High substrate takamaiman.
★ Karfin chiral selectivity.
★ Babban juzu'i.
★ Kadan kayan masarufi.
★ Sharuɗɗan ɗaukar hankali.
★ Abokan Muhalli.
★ Babban aminci.
➢ A al'ada, tsarin amsawa ya kamata ya haɗa da substrate, maganin buffer (pH mafi kyawun amsawa), coenzymes (NAD (H) ko NADP (H)), tsarin farfadowa na coenzyme (misali glucose da glucose dehydrogenase) da ES-ERED.
➢ Duk ES-EREDs ana iya gwada su bi da bi a cikin tsarin amsawa a sama ko tare da ERED Screening Kit (SynKit ERED).
➢ Duk nau'ikan ES-EREDs masu dacewa da yanayi daban-daban masu inganci yakamata a yi nazari akai-akai.
➢ Babban abun da ke ciki ko samfur mai iya hana ayyukan ES-ERED.Duk da haka, ana iya samun sauƙin hanawa ta hanyar ƙari na substrate.
Misali 1 (α, β-unsaturated aldehydes ko ketones)(1):
Misali 2 (α, β-unsaturated carboxylic acid da abubuwan da suka samo asali)(2):
Ci gaba da shekaru 2 ƙasa -20 ℃.
Kada a taɓa hulɗa da matsananciyar yanayi kamar: babban zafin jiki, high / low pH da babban taro kwayoyin kaushi.
1. Lucídio C, Fardelone J, Augusto R, da tal.J.Mol.Catal.B:Enzym., 2004, 29: 41-45.
2. Stueckler C, Hall M, Ehammer H, da tal..Org.Lett, 2007, 9 (26): 5409-5411.
