Cyclohexanone monooxygenase (CHMO)
Takaitaccen Bayani:
Game da Cyclohexanone monooxygenase
ES-CHMO (Cyclohexanone monooxygenase): wani Baeyer Villiger monooxygenase (BVMO) tare da cyclohexanone a matsayin substrate, zai iya oxidize cyclohexanone zuwa cyclohexanol ta amfani da oxygen.
Wani nau'in enzyme ne wanda ya dogara da flavin.Flavin mai oxidized yana raguwa zuwa flavin ta hanyar sake zagayowar rage coenzyme II (NADPH) don iskar oxygen na gaba.Saboda haka, cofactor NADPH ya zama dole a cikin zagayowar flavin.Akwai nau'in samfurin enzyme na CHMO guda 1 kawai (Lamba kamar ES-CHMO101) wanda SyncoZymes ya haɓaka, wanda za'a iya amfani dashi don oxidize cyclohexanone zuwa cyclohexanone.
Nau'in amsawar catalytic:
Wayar hannu/Wechat/WhatsApp: +86-13681683526
Imel:lchen@syncozymes.com
| Enzymes | Lambar samfur | Ƙayyadaddun bayanai |
| Kit ɗin Nuna (SynKit) | Saukewa: ES-CHMO-101 | saitin 1 Ketoreductases, 1 MG kowane 1 abubuwa * 1 MG / abu |
★ High substrate takamaiman.
★ Karfin chiral selectivity.
★ Babban juzu'i.
★ Kadan kayan masarufi.
★ Sharuɗɗan ɗaukar hankali.
★ Abokan Muhalli.
➢ Yawanci, tsarin amsawa yakamata ya haɗa da substrate, maganin buffer, enzyme, coenzyme da tsarin farfadowa na coenzyme (misali glucose da glucose dehydrogenase).
➢ Ya kamata a ƙara CHMO a ƙarshe cikin tsarin amsawa, bayan an daidaita pH da zafin jiki zuwa yanayin amsawa.
Misali 1 (Tsarin cyclohexanol daga cyclohexanol)(1):
Ci gaba da shekaru 2 ƙasa -20 ℃.
Kada a taɓa hulɗa da matsananciyar yanayi kamar: babban zafin jiki, high / low pH da babban taro kwayoyin kaushi.
1. Friso, Marco, Appl Microbiol Biotechnol, 2017, 101 (20): 7557-7565
