Cyclohexanone monooxygenase (CHMO)
Takaitaccen Bayani:
Game da Cyclohexanone monooxygenase
ES-CHMO (Cyclohexanone monooxygenase): Baeyer Villiger monooxygenase (BVMO) tare da cyclohexanone a matsayin substrate, zai iya oxidize cyclohexanone zuwa cyclohexanol ta amfani da iskar oxygen.
Wani nau'in enzyme ne da ke dogara da flavin. Flavin ɗin da aka yi oxidized ana rage shi zuwa flavin ta hanyar zagayowar rage coenzyme II (NADPH) don oxidation na gaba. Saboda haka, cofactor NADPH ya zama dole a cikin zagayowar flavin. Akwai nau'in samfurin enzyme CHMO guda ɗaya kawai (Lambar kamar ES-CHMO101) wanda SyncoZymes ya haɓaka, wanda za'a iya amfani da shi don oxidizing cyclohexanone zuwa cyclohexanone.
Nau'in amsawar catalytic:
Wayar Salula/Wechat/WhatsApp: +86-13681683526
Imel:lchen@syncozymes.com
| Enzymes | Lambar Samfura | Ƙayyadewa |
| Kayan tantancewa (SynKit) | ES-CHMO-101 | saitin Ketoreductases 1, 1 mg kowanne 1 abu * 1mg / abu |
★ Babban takamaiman substrate.
★ Ƙarfin zaɓin chiral.
★ Babban canji.
★ Rage yawan kayayyakin da aka samar.
★ Yanayin amsawa mai sauƙi.
★ Mai kyau ga muhalli.
➢ A al'ada, tsarin amsawar yakamata ya haɗa da substrate, maganin buffer, enzyme, coenzyme da tsarin sake farfaɗo da coenzyme (misali glucose da glucose dehydrogenase).
➢ Ya kamata a ƙara CHMO a ƙarshe a cikin tsarin amsawa, bayan an daidaita pH da zafin jiki zuwa yanayin amsawar.
Misali na 1(Haɗa cyclohexanol daga cyclohexanol)(1):
Ajiye shi a wuri mai sanyi na tsawon shekaru 2 ƙasa da -20 ℃.
Kada a taɓa yin hulɗa da yanayi mai tsanani kamar: zafin jiki mai yawa, pH mai yawa/ƙaranci da kuma yawan sinadarin sinadarai masu narkewa.
1. Friso, Marco, Appl Microbiol Biotechnol, 2017, 101 (20): 7557-7565
