Ketoreductase (KRED)
KRED daga SyncoZymes: Akwai nau'ikan samfuran enzyme na KRED 191 (Lambar kamar ES-KRED-101 ~ ES-KRED-291) wanda SyncoZymes ya haɓaka.SZ-KRED za a iya amfani da ko'ina a regio- da stereoselective rage aldehydes, beta-ketone esters, α-ketone esters da ketones.
Nau'in amsawar catalytic:
| Enzymes | Lambar samfur | Ƙayyadaddun bayanai |
| Enzyme Foda | Enzyme Foda | saitin 187 Ketoreductases, 50 MG kowane 187 abubuwa * 50mg / abu, ko wani adadi |
| Kit ɗin Nuna (SynKit) | Saukewa: ES-KRED-18100 | saitin 181 Ketoreductases, 1 MG kowane 181 abubuwa * 1 MG / abu |
★ High substrate takamaiman.
★ Karfin chiral selectivity.
★ High juyi yadda ya dace.
★ Kadan kayan masarufi.
★ Sharuɗɗan ɗaukar hankali.
★ Abokan Muhalli.
➢ Ya kamata a gudanar da gwajin gwajin enzyme na musamman saboda ƙayyadaddun ƙayyadaddun kayan, kuma a sami wani enzyme wanda ke haifar da maƙasudin maƙasudin tare da mafi kyawun tasirin kuzari.
➢ Kada a taɓa hulɗa da matsanancin yanayi kamar: babban zafin jiki, high / low pH da kwayoyin kaushi tare da babban taro.
➢ Yawanci, tsarin amsawa ya kamata ya haɗa da substrate, maganin buffer, coenzymes (NAD (H) ko NADP (H)), tsarin farfadowa na coenzyme (misali glucose da glucose dehydrogenase).
➢ KRED yakamata a ƙara ƙarshe cikin tsarin amsawa, bayan an daidaita pH da zafin jiki zuwa yanayin amsawa.
➢ Duk nau'ikan KRED suna da mafi kyawun yanayin amsawa daban-daban, don haka kowane ɗayansu yakamata a ƙara yin nazari akai-akai.
Misali 1(1):
Misali 2(2):
1. Li Z, Liu WD, Chen X, da tal.Tetrahedron, 2013, 69, 3561-3564.
2. Ni Y, Li CX, Ma HM, da tal.Appl Microbiol Biotechnol, 2011, 89: 1111-1118.
