Leucine Dehydrogenase (LeuDH)
Takaitaccen Bayani:
Bayani game da Leucine dehydrogenase
Akwai nau'in samfurin enzyme na LeuDH guda ɗaya (Lambar kamar ES-LeuDH-101) wanda SyncoZymes ya ƙirƙira. LeuDH na iya haɓaka ammonia mai rage ammonia na 4-methylbutanoic acid (gishiri) zuwa L-leucine ko kuma oxidation na leucine zuwa 4-methylbutanoic acid. ES-LeuDH kuma na iya haɓaka amination mai rage ammonia na α-keto acid tare da tsari iri ɗaya don shirya abubuwan da suka dace na amino acid. A cikin tsarin catalysis, NADH ya zama dole a matsayin mai jigilar hydrogen.
Nau'in amsawar Leucine dehydrogenasecatalytic
Wayar Salula/Wechat/WhatsApp: +86-13681683526
Imel:lchen@syncozymes.com
| Enzymes | Lambar Samfura | Ƙayyadewa |
| Foda na Enzyme | ES-LeuDH-101 | saitin Monoamine Oxidase 1, 50 mg kowanne 1 abu * 50mg / abu, ko wani adadi |
Ana iya amfani da tsarin amsawa mai zuwa don tunani:
1-10 mg/ml: Substrate
10 mg/ml: Enzyme
10% (v/v): Haɗin sinadarai na halitta (1)
100 mM: Ma'aunin Phosphate (pH7.0)
0.2 mg/ml: NAD+
100 mM: Tsarin Ammonium
10 mg/ml: Tsarin dehydrogenase (FDH)
A saka halayen a cikin na'urar girgiza zafin jiki akai-akai (misali, 30oC, 150rpm) na tsawon awanni 24 ~ 48. A sanya na'urar sanyaya iska a kowace cakuda don tada furotin, ana iya amfani da su wajen nazarin TLC, HPLC ko GC.
Lura (1): Maganin narkewar halitta (kamar DMSO, methanol, acetonitrile, IPA, da sauransu) zaɓi ne, ana amfani da shi don abubuwan da ba sa narkewa.
