Leucine Dehydrogenase (LeuDH)
Takaitaccen Bayani:
Game da Leucine dehydrogenase
Akwai nau'in samfurin enzyme LeuDH guda 1 (Lambar kamar ES-LeuDH-101) wanda SyncoZymes ya haɓaka.LeuDH na iya haifar da raguwar ammonium na 4-methylbutanoic acid (gishiri) zuwa L-leucine ko oxidation na leucine zuwa 4-methylbutanoic acid.ES-LeuDH kuma yana iya haɓaka haɓakar haɓakar α-keto acid tare da tsari iri ɗaya don shirya abubuwan da suka dace da amino acid.A cikin aiwatar da catalysis, NADH ya zama dole a matsayin mai jigilar hydrogen.
Leucine dehydrogenasecatalytic dauki nau'in
Wayar hannu/Wechat/WhatsApp: +86-13681683526
Imel:lchen@syncozymes.com
| Enzymes | Lambar samfur | Ƙayyadaddun bayanai |
| Enzyme Foda | ES-LeuDH-101 | saitin 1 Monoamine Oxidase, 50 MG kowane 1 abubuwa * 50mg / abu, ko wani adadi |
Ana iya amfani da tsarin amsawa mai zuwa don tunani:
1-10 mg/ml: Substrate
10 mg/ml: Enzyme
10% (v/v): Na'ura mai narkewa (1)
100mM: Buffer Phosphate (pH7.0)
0.2 mg/ml: NAD+
100 mM: Ammonium formate
10 mg/ml: Formate dehydrogenase (FDH)
Haɓaka halayen a madaidaicin zafin jiki (misali, 30oC, 150rpm) cikin sa'o'i 24 ~ 48.Centrifuge kowane cakuda don haɓaka furotin, ana iya amfani da su don nazarin TLC, HPLC ko GC.
Lura (1): Maganin halitta (kamar DMSO, methanol, acetonitrile, IPA, da sauransu) zaɓi ne, ana amfani dashi don abubuwan da ba za a iya narkewa ba.
