Transaminase (ATA)
Enzymes: Su ne macromolecular biostimulators, yawancin enzymes sunadarai ne.
Transaminases: Wani nau'in enzymes ne da ke haɓaka amino acid da ke canzawa tsakanin amino acid da keto acid. Transaminases sune manyan enzymes na halitta a cikin haɗin da ba a daidaita ba da kuma ƙudurin launin fata na chiral amines.
Ana iya raba aminotransferase zuwa azuzuwa huɗu bisa ga tsari da tsari: Ⅰ, Ⅱ, Ⅲ da Ⅳ. ω-aminotransferases suna cikin transaminases na aji na II, waɗanda ake amfani da su sosai wajen shirya chiral amines da amino acid na halitta, kamar β-amino acid.
ω-aminotransferases: A mafi yawan lokuta, ω-transaminase yana nufin wani nau'in enzymes, wanda ke canza halayen ammonia mai catalytic ba tare da α-amino acid a matsayin substrate ko samfur ba.
Tsarin haɓaka kuzari:
| Enzymes | Lambar Samfura | Lambar Samfura |
| Foda na Enzyme | ES-ATA-101~ ES-ATA-165 | saitin 65 ω-Transaminases, 50 MG kowanne 65 abubuwa * 50mg / abu, ko wani adadi |
| Kayan tantancewa (SynKit) | ES-ATA-6500 | saitin 65 ω-Transaminases, 1 mg kowanne 65 abubuwa * 1mg / abu |
★ Babban takamaiman substrate.
★ Ƙarfin zaɓin chiral.
★ Ingantaccen aiki na juyawa.
★ Rage yawan kayayyakin da aka samar.
★ Yanayin amsawa mai sauƙi.
★ Mai kyau ga muhalli.
➢ Ya kamata a yi gwajin enzyme don takamaiman substrates saboda takamaiman substrates, kuma a sami enzyme wanda ke haɓaka substrates ɗin da aka nufa tare da mafi kyawun tasirin catalytic.
➢ Kada ka taɓa haɗuwa da yanayi mai tsanani kamar: zafi mai yawa, pH mai yawa/ƙasa da kuma sinadarin sinadarai masu ƙarfi waɗanda ke da yawan amfani.
➢ A al'ada, tsarin amsawar yakamata ya haɗa da substrate, maganin buffer, amino donor (kamar amino acid da 1-phenyl ethylamine) ko receptor (kamar keto acid), coenzyme (PLP), cosolvent (kamar DMSO).
➢ Ya kamata a ƙara ATA a ƙarshe a cikin tsarin amsawa, bayan an daidaita pH da zafin jiki zuwa yanayin amsawar.
➢ Duk nau'ikan ATA suna da yanayi daban-daban na amsawar da ta dace, don haka ya kamata a ƙara yin nazari a kansu.
Misali na 1(haɗa Sitagliptin, haɗakar da ba ta da alaƙa)(1):
Misali na 2 (Mexiletine, Haɗakar ƙudurin motsi tare da haɗakar rashin daidaituwa)(2):
1 Savile CK, Janey JM, Mundorff EC, da sauransu. Kimiyya, 2010, 329(16), 305-309.
2 Koszelewski D, Pressnitz D, Clay D, et al. Haruffa na halitta, 2009,11 (21): 4810-4812.
