Transaminase (ATA)
Enzymes: Su ne macromolecular nazarin halittu catalysts, mafi yawan enzymes sunadaran gina jiki.
Transaminases: Wani nau'in enzymes wanda ke haifar da canja wurin amino tsakanin amino acid da keto acid.Transaminases sune mahimman enzymes na halitta a cikin haɗin asymmetric da ƙudurin tseren chiral amines.
Aminotransferase za a iya raba kashi hudu bisa ga jeri da tsari: Ⅰ, Ⅱ, Ⅲ da Ⅳ.ω-aminotransferases na cikin aji II transaminases, yawanci ana amfani dashi a cikin shirye-shiryen amines na chiral da amino acid marasa dabi'a, irin su β-amino acid.
ω-aminotransferases: A mafi yawan lokuta, ω-transaminase yana nufin wani nau'in enzymes, cewa catalytic ammonia canja wurin halayen ba tare da α-amino acid a matsayin substrate ko samfur.
Na'urar catalytic:
| Enzymes | Lambar samfur | Lambar samfur |
| Enzyme Foda | ES-ATA-101~ ES-ATA-165 | saitin 65 ω-Transaminases, 50 MG kowane 65 abubuwa * 50mg / abu, ko wasu adadi |
| Kit ɗin Nuna (SynKit) | Saukewa: ES-ATA-6500 | saitin 65 ω-Transaminases, 1 MG kowane 65 abubuwa * 1 MG / abu |
★ High substrate takamaiman.
★ Karfin chiral selectivity.
★ High juyi yadda ya dace.
★ Kadan kayan masarufi.
★ Sharuɗɗan ɗaukar hankali.
★ Abokan Muhalli.
➢ Ya kamata a gudanar da gwajin gwajin enzyme na musamman saboda ƙayyadaddun ƙayyadaddun kayan, kuma a sami wani enzyme wanda ke haifar da maƙasudin maƙasudin tare da mafi kyawun tasirin kuzari.
➢ Kada a taɓa hulɗa da matsanancin yanayi kamar: babban zafin jiki, high / low pH da kwayoyin kaushi tare da babban taro.
➢ Yawanci, tsarin amsawa yakamata ya haɗa da substrate, maganin buffer, mai ba da gudummawar amino (kamar amino acid da 1-phenyl ethylamine) ko mai karɓa (kamar keto acid), coenzyme (PLP), cosolvent (kamar DMSO).
➢ ATA yakamata a ƙara ƙarshe cikin tsarin amsawa, bayan an daidaita pH da zafin jiki zuwa yanayin amsawa.
➢ Duk nau'ikan ATA suna da yanayin amsawa daban-daban, don haka kowane ɗayansu yakamata a ƙara yin nazari akai-akai.
Misali 1 (haɗin Sitagliptin, haɗin asymmetric)(1):
Misali 2 (Mexiletine, Haɗin ƙudurin motsi tare da haɗin asymmetric)(2):
1 Savile CK, Janey JM, Mundorff EC, et al.Kimiyya, 2010, 329 (16), 305-309.
2 Koszelewski D, Pressnitz D, Clay D, et al.Haruffa na halitta, 2009,11 (21): 4810-4812.
