Alcohol oxidase (AOX)
Enzymes | Lambar samfur | Ƙayyadaddun bayanai |
Enzyme Foda | ES-AOX-101~ ES-AOX-105 | saitin 5 barasa oxidases, 50 MG kowane 5 abubuwa * 50mg / abu, ko wasu yawa |
Kit ɗin Nuna (SynKit) | Saukewa: ES-AOX-500 | saitin 5 barasa oxidases, 50 MG kowane 5 abubuwa * 50mg / abu, ko wasu yawa |
★ High substrate takamaiman.
★ Babban juzu'i.
★ Kadan kayan masarufi.
★ Sharuɗɗan ɗaukar hankali.
★ Abokan Muhalli.
➢ A al'ada, tsarin amsawa ya kamata ya haɗa da substrate, maganin buffer da ES-AOX, kuma oxygen ya kamata ya kasance.
➢ Duk nau'ikan ES-AOXs masu dacewa da mafi kyawun yanayin amsawa, waɗanda za'a iya yin nazari akai-akai.
➢ Babban abun da ke ciki ko samfur mai iya hana ayyukan ES-AOX.Duk da haka, ana iya sauƙaƙe hanawa ta hanyar ƙara nau'i na substrate.
➢ Tarin H2O2a cikin tsarin zai haifar da rashin aiki na enzyme, wanda za'a iya magance shi sosai ta hanyar amfani da catalase.
Misali 1 (Oxidation na aryl-alcohols)(1):
Misali 2 (Oxidation of fatty alcohols)(2):
Ci gaba da shekaru 2 ƙasa -20 ℃.
Kada a taɓa hulɗa da matsananciyar yanayi kamar: babban zafin jiki, high / low pH da babban taro kwayoyin kaushi.
1. Benen J AE, Sa' nchez-Torres P, Wagemaker M JM, da tal.J Biol Chem, 1998, 273 (14): 7865-7872.
2. Mauersberger S, Drechsler H, Oehme G, da tal.Appl Microbiol Biot, 1992, 37: 66-73.