Barasa mai guba (AOX)
Takaitaccen Bayani:
Game da barasa oxidase
ES-AOXs: Waɗannan enzymes suna iya haɓaka iskar shaka ta kitse ko aryl-alcohols don samar da aldehydes. Suna amfani da iskar shaka ta kwayoyin halitta amma ba sa buƙatar wani abu da aka ƙara a waje, kuma ana samar da hydrogen peroxide.
Akwai nau'ikan samfuran barasa oxidase guda 6 (Lambar da aka fi sani da ES-AOX-101~ES-AOX-106) waɗanda SyncoZymes suka ƙirƙira. ES-AOX101 da ES-AOX102 sun fi son sinadaran aliphatic, ES-AOX103~ ES-AOX105 sun fi son sinadaran aromatic, kuma ES-AOX106 shine cholesterol oxidase. SZ-AOX kayan aiki ne mai amfani don haɓaka iskar shaka ta fatty alcohols ko aryl-alcohols don samar da aldehydes.
Nau'in amsawar catalytic:
Wayar Salula/Wechat/WhatsApp: +86-13681683526
Imel:lchen@syncozymes.com
| Enzymes | Lambar Samfura | Ƙayyadewa |
| Foda na Enzyme | ES-AOX-101~ ES-AOX-105 | saitin barasa oxidases guda 5, 50 mg kowanne 5 * 50mg / abu, ko wani adadi |
| Kayan tantancewa (SynKit) | ES-AOX-500 | saitin barasa oxidases guda 5, 50 mg kowanne 5 * 50mg / abu, ko wani adadi |
★ Babban takamaiman substrate.
★ Babban canji.
★ Rage yawan kayayyakin da aka samar.
★ Yanayin amsawa mai sauƙi.
★ Mai kyau ga muhalli.
➢ A al'ada, tsarin amsawar yakamata ya haɗa da substrate, maganin buffer da ES-AOX, kuma iskar oxygen yakamata ta kasance.
➢ Duk nau'ikan ES-AOXs da suka dace da yanayi daban-daban na amsawar da ta dace, waɗanda za a iya yin nazari a kansu.
➢ Yawan sinadarin substrate ko samfurin da ke ɗauke da shi na iya hana ayyukan ES-AOX. Duk da haka, ana iya rage toshewar ta hanyar ƙara substrate a cikin rukuni.
➢ Tarin H2O2A cikin tsarin zai haifar da rashin aiki na enzyme, wanda za'a iya magance shi ta hanyar amfani da catalase.
Misali na 1(Shan sinadarin aryl-alcohols)(1):
Misali na 2 (Shan sinadarin barasa mai guba)(2):
Ajiye shi a wuri mai sanyi na tsawon shekaru 2 ƙasa da -20 ℃.
Kada a taɓa yin hulɗa da yanayi mai tsanani kamar: zafin jiki mai yawa, pH mai yawa/ƙaranci da kuma yawan sinadarin sinadarai masu narkewa.
1. Benen J AE, Sa' nchez-Torres P, Wagemaker M JM, da tal. J Biol Chem, 1998, 273 (14): 7865-7872.
2. Mauersberger S, Drechsler H, Oehme G, da tal. Appl Microbiol Biot, 1992, 37: 66-73.
