Cytochrome P450 monooxygenase (CYP)
Nau'in amsawar catalytic:
| Enzymes | Kayan tantancewa (SynKit) | Ƙayyadewa |
| Foda na Enzyme | ES-CYP-101~ ES-CYP-108 | saitin Cytochrome P450 Monooxygenases guda 8, 50 MG kowanne. Abubuwa 8 * 50mg/abu, ko wani adadi |
| Kayan tantancewa (SynKit) | ES-CYP-800 | saitin Cytochrome P450 Monooxygenases guda 8, 1mg kowanne abu 8 * 1mg / abu |
★ Faɗaɗɗen bakan substrate.
★ Babban canji.
★ Rage yawan kayayyakin da aka samar.
★ Yanayin amsawa mai sauƙi.
★ Mai kyau ga muhalli.
➢ A al'ada, tsarin amsawar yakamata ya haɗa da substrate, maganin buffer (Mafi kyawun pH na amsawar enzyme), coenzyme (NAD(H) ko NADP(H)), tsarin sake farfaɗo da coenzyme (misali glucose da glucose dehydrogenase) da kuma tsarin sake farfaɗo da coenzyme na ES-CYP. za a iya maye gurbinsu da hydrogen peroxide a cikin tsarin sake farfaɗo da wani ɓangare.
➢ Ya kamata a yi nazarin dukkan nau'ikan ES-CYPs da suka dace da yanayin amsawar da ya dace daban-daban.
➢ Yawan sinadarin substrate ko samfurin da ke ɗauke da shi na iya hana ayyukan ES-CYP. Duk da haka, ana iya rage toshewar ta hanyar ƙara substrate a cikin rukuni.
Misali na 1(1):
Misali na 2(2):
Misali na 3(3):
Misali na 4(4):
Ajiye shi a wuri mai sanyi na tsawon shekaru 2 ƙasa da -20 ℃.
Kada a taɓa yin hulɗa da yanayi mai tsanani kamar: zafin jiki mai yawa, pH mai yawa/ƙaranci da kuma yawan sinadarin sinadarai masu narkewa.
1. Zaretzki J, Matlock M, da Swamadass S JJ Chem. Inf. Model, 2013, 53, 3373–3383.
2. Gannett P M., Kabulski J, Perez F A., da tal. J. Am. Chem. So, 2006, 128 (26), 8374-8375.
3. Cryle M J., Matovic N J., da De Voss J J. Org. Lett., 2003, 5 (18), 3341–3344.
4. Kawauchi, H., Sasaki, J., Adachi, T., e tal. Biochim. Biophys. Dokar, 1994, 1219, 179.
5. Yasutake, Y., Fujii, Y.; Cheon, WK da tal. Acta Crystallogr. 2009, 65, 372.
