Cytochrome P450 monooxygenase (CYP)

Takaitaccen Bayani:

Bayanin CYP

ES-CYPs: Wani aji na redox enzymes tare da mercaptan-heme a matsayin cibiyar aiki don haɓaka iskar oxygen.Su ne m oxygenases kuma yana buƙatar heme don kunna oxygen.

Cytochrome P450 monooxygenase catalyze da hadawan abu da iskar shaka, epioxidation da hydroxylation na alkyl, hydroxylation da hadawan abu da iskar shaka na ammonia, hadawan abu da iskar shaka na sulfur, dealkylation (oxygen, sulfur, ammonia), oxidative dehydrogenation, deamination da dehalogenation.
Akwai nau'ikan samfuran cytochrome P450 monooxygenase (Lambar kamar ES-CYP-101-ES-CYP-108) wanda SyncoZymes ya haɓaka.

Wayar hannu/Wechat/WhatsApp: +86-13681683526

Imel:lchen@syncozymes.com

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Cikakken Bayani

Tags samfurin

Game da CYP:

Nau'in amsawar catalytic:

Bayanin samfur:

Enzymes	Kit ɗin Nuna (SynKit)	Ƙayyadaddun bayanai
Enzyme Foda	ES-CYP-101~ ES-CYP-108	saitin 8 Cytochrome P450 Monooxygenases, 50 MG kowane 8 abubuwa * 50mg / abu, ko wani adadi
Kit ɗin Nuna (SynKit)	Saukewa: ES-CYP-800	saitin 8 Cytochrome P450 Monooxygenases, 1 MG kowane 8 abubuwa * 1 MG / abu

Amfani:

★ Broad substrate bakan.
★ Babban juzu'i.
★ Kadan kayan masarufi.
★ Sharuɗɗan ɗaukar hankali.
★ Abokan Muhalli.

Umarnin don amfani:

➢ A al'ada, tsarin amsawa ya kamata ya haɗa da substrate, maganin buffer (Mafi kyawun halayen pH na enzyme), coenzyme (NAD (H) ko NADP (H)), tsarin farfadowa na coenzyme (misali glucose da glucose dehydrogenase) da ES-CYP.coenzyme da tsarin farfadowa na coenzyme za a iya maye gurbinsu ta hanyar hydrogen peroxide a cikin tsarin dauki bangare.
➢ Duk nau'ikan ES-CYPs masu dacewa da yanayi daban-daban masu inganci yakamata a yi nazari akai-akai.
➢ Babban abun da ke ciki ko samfur mai iya hana ayyukan ES-CYP.Duk da haka, ana iya sauƙaƙe hanawa ta hanyar ƙara nau'i na substrate.

Misalai na aikace-aikacen:

Misali 1⁽¹⁾:

Misali 2⁽²⁾:

Misali 3⁽³⁾:

Misali 4⁽⁴⁾:

Ajiya:

Ci gaba da shekaru 2 ƙasa -20 ℃.

Hankali:

Kada a taɓa hulɗa da matsananciyar yanayi kamar: babban zafin jiki, high / low pH da babban taro kwayoyin kaushi.

Magana:

1. Zaretzki J, Matlock M, da Swamidass S JJ Chem.Inf.Model, 2013, 53, 3373-3383.
2. Gannett P M., Kabulski J, Perez F A., da tal.J. Am.Chem.Soc., 2006, 128 (26), 8374-8375.
3. Cryle M J., Matovic N J., da De Voss J J. Org.Lett., 2003, 5 (18), 3341-3344.
4. Kawauchi, H., Sasaki, J., Adachi, T., e tal.Biochim.Biophys.Dokar, 1994, 1219, 179.
5. Yasutake, Y., Fujii, Y.;Cheon, WK da tal.Acta Crystallogr.2009, 65, 372.