Phenylalanine dehydrogenase (PDH)
Takaitaccen Bayani:
Game da Phenylalanine dehydrogenase
ES-PDH: aji na oxidoreductase.Ingantacciyar amsawa na iya haifar da deamination oxidative deamination na L phenylalanine gishiri a gaban NAD don samar da phenylpyruvate gishiri, da kuma mayar da martani zai iya catalyze da kira na daidai amino acid gishiri daga phenylpyruvate gishiri.Syncozymes sun haɓaka abubuwa 8 na phenylalanine dehydrogenase (lamba ES-PDH-101~ES-PDH-108).Ana iya amfani da ES-PDH don shirya gishirin phenylalanine ko gishirin amino acid daidai daga gishiri na phenylpyruvic acid ko abubuwan da aka samo asali.
Nau'in amsawar catalytic:
Wayar hannu/Wechat/WhatsApp: +86-13681683526
Imel:lchen@syncozymes.com
| Enzymes | Lambar samfur | Ƙayyadaddun bayanai |
| Enzyme Foda | ES-PDH-101~ ES-PDH-108 | saitin 8 Nitrile Reductase, 50 MG kowane 8 abubuwa * 50mg / abu, ko wani adadi |
★ High substrate takamaiman.
★ Karfin chiral selectivity.
★ Babban juzu'i.
★ Kadan kayan masarufi.
★ Sharuɗɗan ɗaukar hankali.
★ Abokan Muhalli.
➢ A al'ada, tsarin amsawa ya kamata ya haɗa da substrate, maganin buffer, enzyme, coenzyme (NAD (H)), tsarin farfadowa na coenzyme (misali ammonium formate da formate dehydrogenase).
➢ Kowane nau'i na ES-PDH wanda ya dace da yanayi daban-daban mafi dacewa, wanda za'a iya yin nazari akai-akai idan an buƙata.
➢ Ya kamata a ƙara ES-PDH a ƙarshe cikin tsarin amsawa don kula da aikin.
Misali 1 (Biosynthesis na abubuwan phenylalanine daga abubuwan phenylpyruvate)(1):
Ci gaba da shekaru 2 ƙasa -20 ℃.
Kada a taɓa hulɗa da matsananciyar yanayi kamar: babban zafin jiki, high / low pH da babban taro kwayoyin kaushi.
1. Francesca Paradisi, Stuart Collins, et al.Jaridar Biotechnology, 2007, 128, 408-411.
