Haidominase (HAM)
Takaitaccen Bayani:
Game da Hydroaminase
Akwai nau'ikan samfurin enzyme na HAM guda biyu (Lambar kamar ES-HAM-101~ ES-HAM-102) wanda SyncoZymes ya ƙirƙira. HAM na iya haɓaka ammonia na enoic acid ko abubuwan da suka samo asali don samar da chiral amino acid. Ana iya amfani da HAM don haɗa chiral amino acid (ko chiral amines) daga enoic acid (ko alkenes). Mai ba da gudummawar ammonia, kamar ruwan ammonia ko gishirin ammonium, ya zama dole.
Nau'in amsawar sinadarin hydroaminase catalytic
Wayar Salula/Wechat/WhatsApp: +86-13681683526
Imel:lchen@syncozymes.com
| Enzymes | Lambar Samfura | Ƙayyadewa |
| Foda na Enzyme | ES-HAM-101~ ES-HAM-102 | saitin Nuclosidephosphoryalse guda 2, 50 MG kowanne abu 2 * 50mg / abu, ko wani adadi |
Ana iya amfani da tsarin amsawa mai zuwa don tunani:
1-10 mg/ml: Substrate
10 mg/ml: Enzyme
10% (v/v): Haɗin sinadarai na halitta (1)
10% (v/v): Ammoniya
A daidaita pH zuwa 9.0 kafin a ƙara enzyme, sannan a ƙara enzyme a ƙarshe. A saka halayen a cikin injin girgiza zafin jiki akai-akai (misali, 30oC, 150rpm) na tsawon awanni 24 ~ 48. A naɗe kowanne cakuda a cikin na'urar sanyaya iska don yaɗa furotin, ana iya amfani da sinadarin da ke cikinsa don nazarin TLC, HPLC ko GC.
Lura (1): Maganin narkewar halitta (kamar DMSO, methanol, acetonitrile, IPA, da sauransu) zaɓi ne, ana amfani da shi don abubuwan da ba sa narkewa.
