Nitrile reductase (NRED)
Takaitaccen Bayani:
Game da Nitrile reductase
Akwai nau'in samfurin enzyme NRED guda 3 (Lambar kamar ES-NRED101 ~ ES-NRED103) wanda SyncoZymes ya haɓaka.NRED na iya haifar da raguwar rukunin nitrile zuwa amin.A cikin aiwatar da catalysis, ana buƙatar NADPH azaman jigilar hydrogen.
Nitrile reductasecatalytic dauki nau'in
Wayar hannu/Wechat/WhatsApp: +86-13681683526
Imel:lchen@syncozymes.com
| Enzymes | Lambar samfur | Ƙayyadaddun bayanai |
| Ƙayyadaddun bayanai | ES- NRED -101~ ES- NRED -103 | saitin 3 Nitrile Reductase, 50 MG kowane 3 abubuwa * 50mg / abu, ko wani adadi |
Ana iya amfani da tsarin amsawa mai zuwa don tunani:
1-10 mg / ml substrate
10 mg / ml enzyme
10% (v/v) Na'ura mai narkewa (1)
100 mm Phosphate buffer (pH7.0)
0.2 mg/ml NADP+
Glucose 10 mg/ml
2 mg/ml Glucose dehydrogenase (GDH)
Haɓaka halayen a madaidaicin zafin jiki (misali, 30oC, 150rpm) cikin sa'o'i 24 ~ 48.Centrifuge kowane cakuda don haɓaka furotin, ana iya amfani da su mafi girma don nazarin TLC, HPLC ko GC.
Lura (1): Maganin halitta (kamar DMSO, methanol, acetonitrile, IPA, da sauransu) zaɓi ne, ana amfani dashi don abubuwan da ba za a iya narkewa ba.
