Hydroaminase (HAM)
Takaitaccen Bayani:
Game da Hydroaminase
Akwai nau'ikan samfuran enzyme na HAM guda biyu (Lambar kamar ES-HAM-101 ~ ES-HAM-102) wanda SyncoZymes ya haɓaka.HAM na iya haifar da ammonium na enoic acid ko abubuwan da suka samo asali don samar da amino acid na chiral.Ana iya amfani da HAM don haɗa amino acid na chiral (ko amines chiral) daga enoic acid (ko alkenes).Mai ba da gudummawar ammonia, kamar ruwan ammonia ko gishiri ammonium, ya zama dole.
Hydroaminase catalytic dauki nau'in
Wayar hannu/Wechat/WhatsApp: +86-13681683526
Imel:lchen@syncozymes.com
| Enzymes | Lambar samfur | Ƙayyadaddun bayanai |
| Enzyme Foda | ES-HAM-101~ ES-HAM-102 | wani sa na 2 Nuclosidephosphoryalse, 50 MG kowane 2 abubuwa * 50mg / abu, ko wasu yawa |
Ana iya amfani da tsarin amsawa mai zuwa don tunani:
1-10 mg/ml: Substrate
10 mg/ml: Enzyme
10% (v/v): Na'ura mai narkewa (1)
10% (v/v): Ammoniya
Daidaita pH zuwa 9.0 kafin ƙara enzyme, kuma ya kamata a ƙara enzyme a ƙarshe.Haɓaka halayen a madaidaicin zafin jiki (misali, 30oC, 150rpm) cikin sa'o'i 24 ~ 48.Centrifuge kowane cakuda don haɓaka furotin, ana iya amfani da su mafi girma don nazarin TLC, HPLC ko GC.
Lura (1): Maganin halitta (kamar DMSO, methanol, acetonitrile, IPA, da sauransu) zaɓi ne, ana amfani dashi don abubuwan da ba za a iya narkewa ba.
