Nitrilase (NIT)
Takaitaccen Bayani:
Game da Nitrilase
ES-NITs: Yana canza rukunin cyanide kai tsaye zuwa rukunin carboxyl, don shirya sinadarin carboxylic acid, wanda ake amfani da shi sosai a fannin magani, kare muhalli, masana'antar sinadarai, ƙarin abinci, yadi, samar da aikin gona da sauran fannoni.
Akwai nau'ikan samfuran Nitrilase (NIT) guda 40 (Lambar a matsayin ES-NIT-101~ES-NIT-140) waɗanda SyncoZymes suka ƙirƙira. ES-NIT kayan aiki ne mai amfani don haɓaka haɗakar chiral carboxylic acid ta hanyar hydrolysis na regio- da stereoselective na nitriles iri-iri na aliphatic da aromatic.
Nau'in amsawar catalytic:
Wayar Salula/Wechat/WhatsApp: +86-13681683526
Imel:lchen@syncozymes.com
| Enzymes | Lambar Samfura | Ƙayyadewa |
| Foda na Enzyme | ES-NIT-101~ ES-NIT-140 | saitin Nitrilase 40, 50 MG kowanne 40 * 50mg / abu, ko wani adadi |
| Kayan tantancewa (SynKit) | ES-NIT-4000 | saitin Nitrilase 40, 1 mg kowanne 40 abubuwa * 1mg / abu |
★ Babban takamaiman substrate.
★ Babban canji.
★ Rage yawan kayayyakin da aka samar.
★ Yanayin amsawa mai sauƙi.
★ Mai kyau ga muhalli.
➢ A al'ada, tsarin amsawar yakamata ya haɗa da substrate, maganin buffer (mafi kyawun amsawar pH) da ES-NIT.
➢ Ana iya gwada duk ES-NITs bi da bi a cikin tsarin amsawar da ke sama ko tare da NIT Screening Kit (SynKit NIT).
➢ Ya kamata a yi nazarin dukkan nau'ikan ES-NITs da suka dace da yanayin amsawar da ya dace daban-daban.
➢ Yawan sinadarin substrate ko samfurin da ke ɗauke da shi na iya hana ayyukan ES-NIT. Duk da haka, ana iya rage wannan hanawa ta hanyar ƙara sinadarin substrate a cikin rukuni.
Misali na 1 (Haɗa sinadarin nicotinic acid)(1):
Misali na 2(Haɗin (R)-(-)-acid mandelic)(2):
Misali na 3 (Haɗa baclofen)(3):
Misali na 4 (Haɗa glycolic acid)(4):
Ajiye shi a wuri mai sanyi na tsawon shekaru 2 ƙasa da -20 ℃.
Kada a taɓa yin hulɗa da yanayi mai tsanani kamar: zafin jiki mai yawa, pH mai yawa/ƙaranci da kuma yawan sinadarin sinadarai masu narkewa.
1 Mathew CD, Nagasawa T, Kobayashi M, da sauransu. Appl. Environ. Microbiol, 1988, 54(4): 1030.
2 Yamamoto K, Oishi K, Fujimatsu I, e tal. App. Muhalli. Microbiol, 1991, 57 (10): 3028.
3 Xu MZ, Ren J, Gong JS, da tal. Chin J. Biotechnol, 2013, 29 (1): 31.
4 Wu S, Fogiel AJ, Petrillo KL, da tal. Biotechnol. Bioeng., 2008, 99 (3): 717.
