Nitrilase (NIT)
Takaitaccen Bayani:
Game da Nitrilase
ES-NITs: Kai tsaye canza ƙungiyar cyanide zuwa ƙungiyar carboxyl, don shirya acid carboxylic, wanda ake amfani da shi sosai a magani, kariyar muhalli, masana'antar sinadarai, ƙari na abinci, yadi, samar da noma da sauran fannoni.
Akwai nau'ikan samfuran Nitrilase (NIT) iri 40 (Lambar kamar ES-NIT-101~ES-NIT-140) wanda SyncoZymes ya haɓaka.ES-NIT kayan aiki ne mai amfani don haɓaka haɗakar acid carboxylic na chiral ta hanyar regio- da stereoselective hydrolysis na kewayon aliphatic da nitriles aromatic.
Nau'in amsawar catalytic:
Wayar hannu/Wechat/WhatsApp: +86-13681683526
Imel:lchen@syncozymes.com
| Enzymes | Lambar samfur | Ƙayyadaddun bayanai |
| Enzyme Foda | ES-NIT-101~ ES-NIT-140 | saitin 40 Nitrilase, 50 MG kowane 40 abubuwa * 50mg / abu, ko wani adadi |
| Kit ɗin Nuna (SynKit) | Saukewa: ES-NIT-4000 | saitin 40 Nitrilase, 1 MG kowane 40 abubuwa * 1 MG / abu |
★ High substrate takamaiman.
★ Babban juzu'i.
★ Kadan kayan masarufi.
★ Sharuɗɗan ɗaukar hankali.
★ Abokan Muhalli.
➢ Yawanci, tsarin amsawa yakamata ya haɗa da substrate, maganin buffer (mafi kyawun amsa pH) da ES-NIT.
➢ Duk ES-NITs ana iya gwada su bi da bi a cikin tsarin amsawa a sama ko tare da Kit ɗin Screening NIT (SynKit NIT).
➢ Duk nau'ikan ES-NITs masu dacewa da mafi kyawun yanayin amsawa yakamata a yi nazari akai-akai.
➢ Babban abun da ke ciki ko samfur mai iya hana ayyukan ES-NIT.Duk da haka, ana iya samun sauƙin hanawa ta hanyar ƙari na substrate.
Misali 1 (Hanyoyin nicotinic acid)(1):
Misali 2 (Harkokin (R)-(-)mandelic acid)(2):
Misali 3 (Hanyoyin Baclofen)(3):
Misali na 4 (Hanyoyin glycolic acid)(4):
Ci gaba da shekaru 2 ƙasa -20 ℃.
Kada a taɓa hulɗa da matsananciyar yanayi kamar: babban zafin jiki, high / low pH da babban taro kwayoyin kaushi.
1 Mathew CD, Nagasawa T, Kobayashi M, e tal.Appl.Muhalli.Microbiol, 1988, 54 (4): 1030.
2 Yamamoto K, Oishi K, Fujimatsu I, e tal.Appl.Muhalli.Microbiol, 1991, 57 (10): 3028.
3 Xu MZ, Ren J, Gong JS, da tal.ChinJ. Biotechnol, 2013, 29 (1): 31.
4 Wu S, Fogiel AJ, Petrillo KL, da tal.Biotechnol.Bioeng., 2008, 99 (3): 717.
