Nitrile hydratase (NHT)
Takaitaccen Bayani:
Game da ES-NHTs
ES-NHTs: nau'in metalloenzyme, wanda ke ba da izinin nitriles a cikin daidaitaccen amide na farko.Ana amfani da su sosai ba kawai don haɗin amino acid, amides, acid carboxylic acid da abubuwan da suka samo asali ba, har ma don shirye-shiryen magungunan chiral.Akwai nau'ikan nitrile hydratases guda 24 waɗanda SyncoZymes suka haɓaka (Lambar kamar ES-NHT-101-ES-NHT-124).
Nau'in amsawar catalytic:
Wayar hannu/Wechat/WhatsApp: +86-13681683526
Imel:lchen@syncozymes.com
| Enzymes | Lambar samfur | Ƙayyadaddun bayanai |
| Enzyme Foda | ES-NHT-101~ ES-NHT-124 | saitin 24 Nitrile Hydratases, 50 MG kowane 24 abubuwa * 50mg / abu, ko wani adadi |
| Kit ɗin Nuna (SynKit) | Saukewa: ES-NHT-2400 | saitin 24 Nnitrile Hydratases, 1 MG kowane 24 abubuwa * 1 MG / abu |
★ High substrate takamaiman.
★ Karfin chiral selectivity.
★ Babban juzu'i.
★ Kadan kayan masarufi.
★ Sharuɗɗan ɗaukar hankali.
★ Abokan Muhalli.
➢ Yawanci, tsarin amsa ya kamata ya haɗa da substrate, maganin buffer (pH mafi kyawun amsa) da ES-NHT.
➢ Duk ES-NHTs ana iya gwada su bi da bi a cikin tsarin amsawa a sama ko tare da Kit ɗin Screening NHT (SynKit NHT).
➢ Duk nau'ikan ES-NHTs masu dacewa da yanayi daban-daban masu inganci yakamata a yi nazari akai-akai.
➢ Babban abun da ke ciki ko samfur mai iya hana ayyukan ES-NHT.Koyaya, ana iya samun sauƙin hanawa ta hanyar ƙara juzu'i, haɗawa tare da amidases ko haɓakar samfur.
Misali 1 (hade tare da amidase)(1):
Misali 2(2):
Ci gaba da shekaru 2 ƙasa -20 ℃.
Kada a taɓa hulɗa da matsananciyar yanayi kamar: babban zafin jiki, high / low pH da babban taro kwayoyin kaushi.
1 Vojtech V, Ludmila M, Alicja BV, da tal.J Mol Catal B-enzym, 2011, 71: 51-55.
2 Gary W.B, Thomas G, Christopher B.M, da kuma tal.Tetrahedron lett, 2010, 51: 1639-1641.
