Benzaldehyde lyase (BAL)
★ High substrate takamaiman.
★ Karfin chiral selectivity.
★ Babban juzu'i.
★ Kadan kayan masarufi.
★ Sharuɗɗan ɗaukar hankali.
★ Abokan Muhalli.
➢ Yawanci, tsarin amsawa ya kamata ya haɗa da substrate, maganin buffer, enzyme, ThDP, Mg2+.
➢ BAL yakamata a ƙara ƙarshe zuwa tsarin amsawa, bayan an daidaita pH da zafin jiki zuwa yanayin amsawa.
Misali 1 (Hadarin N-musanyawa 1,2 amino alcohols daga aldehydes)(1):
Ci gaba da shekaru 2 ƙasa -20 ℃.
Kada a taɓa hulɗa da matsananciyar yanayi kamar: babban zafin jiki, high / low pH da babban taro kwayoyin kaushi.
1. Li Y, Hu N, Xu Z, da dai sauransu.Angewandte Chemie International Edition, 2022, 61(17): e202116344.
Ku rubuta sakonku anan ku aiko mana