Benzaldehyde lyase (BAL)
Takaitaccen Bayani:
Game da Benzaldehyde lyase
ES-BAL (Benzaldehyde lyase): wani nau'in enzyme ne wanda ke haɓaka hydroxymethylation na aromatic da aliphatic aldehydes tare da formaldehyde don samar da α-Hydroxymethyl ketone mai dacewa. BAL shine enzyme wanda ya dogara da thiamine diphosphate (ThDP). Akwai nau'in samfurin enzyme BAL guda ɗaya kawai (Lambar kamar ES-BAL101) wanda SyncoZymes ya ƙirƙira, wanda za'a iya amfani dashi don hydroxymethylation na aromatic da aliphatic aldehydes.
Nau'in amsawar catalytic:
Wayar Salula/Wechat/WhatsApp: +86-13681683526
Imel:lchen@syncozymes.com
★ Babban takamaiman substrate.
★ Ƙarfin zaɓin chiral.
★ Babban canji.
★ Rage yawan kayayyakin da aka samar.
★ Yanayin amsawa mai sauƙi.
★ Mai kyau ga muhalli.
➢ A al'ada, tsarin amsawar yakamata ya haɗa da substrate, maganin buffer, enzyme, ThDP, Mg2+.
➢ Ya kamata a ƙara BAL a ƙarshe a cikin tsarin amsawa, bayan an daidaita pH da zafin jiki zuwa yanayin amsawar.
Misali na 1 (Haɗar alcohols amino 1,2 da aka maye gurbinsu da N daga aldehydes)(1):
Ajiye shi a wuri mai sanyi na tsawon shekaru 2 ƙasa da -20 ℃.
Kada a taɓa yin hulɗa da yanayi mai tsanani kamar: zafin jiki mai yawa, pH mai yawa/ƙaranci da kuma yawan sinadarin sinadarai masu narkewa.
1. Li Y, Hu N, Xu Z, da dai sauransu. Angewandte Chemie International Edition, 2022, 61(17): e202116344.
