Nucloside phosphoryalse (NP)

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Game da nucleoside phosphoryalse

Akwai nau'ikan samfuran enzyme NP guda 3 (Lambar kamar ES-NP-101 ~ ES-NP-103) wanda SyncoZymes ya haɓaka.ES-NP-101 shine purine nucleoside phosphorylase, ES-NP-102 da ES-NP-103 sune pyrimidine nucleoside phosphorylase.Nucleosidephosphorylase zai iya rushe nucleosides zuwa sansanonin da pentose phosphate.Nucleoside phosphorylase za a iya raba zuwa purine nucleoside phosphorylase da pyrimidine nucleoside phosphorylase bisa ga fifiko na nucleoside tushe.Purine nucleoside phosphorylase iya metabolize adenosine zuwa adenine, inosine zuwa hypoxanthine, guanosin zuwa guanine, da kuma samar da ribose phosphate a lokaci guda.Pyrimidine nucleoside phosphorylase na iya metabolize uridine zuwa uracil kuma ya samar da ribose phosphate.

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Nau'in amsawar Nucloside phosphoryalse:

Bayanin samfur:

Enzymes	Lambar samfur	Ƙayyadaddun bayanai
Enzyme Foda	ES-NP-101~ ES-NP-103	wani sa na 3 Nuclosidephosphoryalse, 50 MG kowane 3 abubuwa * 50mg / abu, ko wasu yawa

Hanyar nuna halayen Nucloside phosphoryalse:

Ana iya amfani da tsarin amsawa mai zuwa don tunani:
1-10 MG / ml
10 mg / ml enzyme
100 mm Phosphate buffer (pH7.0)
Haɗa halayen a madaidaicin zafin jiki (misali, 30oC, 150rpm) a cikin sa'o'i 12 ~ 24.Centrifuge kowane cakuda don haɓaka furotin, ana iya amfani da su don nazarin TLC, HPLC ko GC.