Epoxide hydrolase (EH)
Takaitaccen Bayani:
Game da epoxide hydrolase
EH shine ether hydrolase wanda ke haɓaka hydrolysis na stereoselective na epoxides na racemic don samar da epoxides 1, 2-diol da masu aiki a ido, waɗanda za a iya amfani da su don magance chiral na epoxides ko shirya chiral diols. Ana amfani da su sosai a magani da sauran fannoni. Akwai nau'ikan samfuran epoxide hydrolases guda 3 (Lambar kamar ES-EH-101～ES-EH-103) waɗanda SyncoZymes suka haɓaka. ES-EH kayan aiki ne mai amfani don haɓaka haɗakar chiral alcohols ta hanyar hydrolysis na aliphatic da aromatic epoxides.
Nau'in amsawar catalytic:
Wayar Salula/Wechat/WhatsApp: +86-13681683526
Imel:lchen@syncozymes.com
| Enzymes | Lambar Samfura | Ƙayyadewa |
| Foda na Enzyme | ES-EH-101~ ES-EH-103 | saitin Epoxide Hydrolases guda 3, 50 mg kowanne 3 * 50mg / abu, ko wani adadi |
| Kayan tantancewa (SynKit) | ES-EH-300 | saitin Epoxide Hydrolases guda 3, 1 mg kowanne abu 3 * 1mg / abu |
★ Babban takamaiman substrate.
★ Ƙarfin zaɓin chiral.
★ Babban canji.
★ Rage yawan kayayyakin da aka samar.
★ Yanayin amsawa mai sauƙi.
★ Mai kyau ga muhalli.
➢ A al'ada, tsarin amsawar yakamata ya haɗa da substrate, maganin buffer (mafi kyawun amsawar pH) da ES-EH.
➢ Ya kamata a yi nazarin dukkan nau'ikan ES-EHs da suka dace da yanayin amsawar da ya dace daban-daban.
➢ Yawan sinadarin substrate ko samfurin da ke ɗauke da shi na iya hana ayyukan ES-EH. Duk da haka, ana iya rage toshewar ta hanyar ƙara substrate a cikin rukuni.
Misali na 1(1):
Ajiye shi a wuri mai sanyi na tsawon shekaru 2 ƙasa da -20 ℃.
Kada a taɓa yin hulɗa da yanayi mai tsanani kamar: zafin jiki mai yawa, pH mai yawa/ƙaranci da kuma yawan sinadarin sinadarai masu narkewa.
1. Kim YH, Lee I, Choi SH, da tal. J Mol Catal B-Enzym, 2013, 89: 48-51.
