Esterase&Lipase (PLE&CALB)
Akwai nau'ikan samfuran enzyme PLE guda 26 (Lambar kamar ES-PLE-101～ES-PLE-126) wanda SyncoZymes ya haɓaka.Ana iya amfani da ES-PLE don hydrolysis na aliphatic da ester mahadi, ko regioselective da stereoselective ƙuduri don hada chiral acid da abubuwan da suka samo asali.
Nau'in amsawar catalytic:
★ High substrate takamaiman.
★ Karfin chiral selectivity.
★ Babban juzu'i.
★ Kadan kayan masarufi.
★ Sharuɗɗan ɗaukar hankali.
★ Abokan Muhalli.
➢ A al'ada, tsarin amsa ya kamata ya haɗa da substrate, maganin buffer da ES-PLE.Ana aiwatar da esterification na wasu ES-PLE a cikin tsarin kwayoyin halitta.
➢ Duk nau'ikan ES-PLEs da suka dace da yanayi daban-daban na amsawa yakamata a yi nazari akai-akai.
Misali 1 (Biosynthesis na Pregabalin Intermediate)(1):
Misali 2(2):
Misali 3(3):
Misali 4(4):
Ci gaba da shekaru 2 ƙasa -20 ℃.
Kada a taɓa haɗuwa da matsanancin yanayi kamar: babban zafin jiki, high/ƙananan pH.
1. Xu FX, Chen SY, Xu G, da tal.Appl.Biotechnol Bioproc E, 1988, 54 (4): 1030.
2. Huang, FC, Lee, LF, Mittal, RSD da tal.J. Am.Chem.Soc, 1975, 97, 4144.
3. Kielbasinski, P., Goralczyk, P., Mikolajczyk, M., e tal.Synlett, 1994, 127.
4. Gais, HJ, Griebel, C., Buschmann, H. Tetrahedron: Asymmetry 2000, 11, 917
