Esterase da Lipase (PLE&CALB)
Akwai nau'ikan samfuran enzyme na PLE guda 26 (Lambar kamar ES-PLE-101～ES-PLE-126) waɗanda SyncoZymes suka ƙirƙira. Ana iya amfani da ES-PLE don hydrolysis na mahaɗan aliphatic da ester, ko kuma regioselective da stereoselective resolution don haɗa chiral acid da abubuwan da suka samo asali.
Nau'in amsawar catalytic:
★ Babban takamaiman substrate.
★ Ƙarfin zaɓin chiral.
★ Babban canji.
★ Rage yawan kayayyakin da aka samar.
★ Yanayin amsawa mai sauƙi.
★ Mai kyau ga muhalli.
➢ A al'ada, tsarin amsawar yakamata ya haɗa da substrate, maganin buffer da ES-PLE. Ana aiwatar da esterification na wasu ES-PLEs a cikin matakin halitta.
➢ Ya kamata a yi nazarin dukkan nau'ikan ES-PLEs da suka dace da yanayin amsawar da ya dace daban-daban.
Misali na 1 (Biosynthesis na Pregabalin Intermediate)(1):
Misali na 2(2):
Misali na 3(3):
Misali na 4(4):
Ajiye shi a wuri mai sanyi na tsawon shekaru 2 ƙasa da -20 ℃.
Kada a taɓa yin hulɗa da yanayi mai tsanani kamar: yanayin zafi mai yawa, pH mai yawa/ƙaranci.
1. Xu FX, Chen SY, Xu G, da sauransu. Appl. Biotechnol Bioproc E, 1988, 54(4): 1030.
2. Huang, FC, Lee, LF, Mittal, RSD da tal. J. Am. Chem.Soc, 1975, 97, 4144.
3. Kielbasinski, P., Goralczyk, P., Mikolajczyk, M., e tal. Synlett, 1994, 127.
4. Gais, HJ, Griebel, C., Buschmann, H. Tetrahedron: Asymmetry 2000, 11, 917
