CALB mara motsi

1. Nau'in Reactor
Enzyme da ba a iya motsi yana da amfani ga kettle batch reactor da kafaffen gado mai ci gaba da kwarara reactor.Ya kamata a lura don kauce wa murƙushewa saboda ƙarfin waje yayin ciyarwa ko cikawa.
2. Reaction pH, zazzabi da sauran ƙarfi
Ya kamata a ƙara enzyme mara motsi na ƙarshe, bayan an ƙara wasu kayan kuma an narkar da su, kuma an daidaita pH.
Idan amfani da substrate ko samuwar samfur zai haifar da canjin pH yayin amsawa, yakamata a ƙara isasshen buffer zuwa tsarin amsawa, ko kuma yakamata a kula da daidaita pH yayin amsawar.
A cikin kewayon haƙurin zafin jiki na CALB (a ƙasa da 60 ℃), ƙimar juyi ya karu tare da haɓakar zafin jiki.A cikin amfani mai amfani, yakamata a zaɓi zafin amsawa gwargwadon kwanciyar hankali na ƙasa ko samfur.
Gabaɗaya, halayen ester hydrolysis ya dace a cikin tsarin lokaci mai ruwa-ruwa, yayin da ester kira amsa ya dace a cikin tsarin zamani.Maganin kwayoyin halitta na iya zama ethanol, tetrahydrofuran, n-hexane, n-heptane da toluene, ko kuma mai dacewa gauraye.Don amsawa a cikin wasu kaushi na halitta, ana iya ƙara 3% ruwa don inganta tasirin amsawa.
3. Sake amfani da rayuwar sabis na CALB
A ƙarƙashin yanayin da ya dace, CALB za a iya dawo da shi kuma a sake amfani da shi, kuma takamaiman lokutan aikace-aikacen sun bambanta da ayyuka daban-daban.
Idan CALB ɗin da aka kwato ba a sake amfani da shi ba kuma yana buƙatar adanawa bayan farfadowa, yana buƙatar wanke shi kuma a bushe kuma a rufe shi a 2-8 ℃.
Bayan zagaye da yawa na sake amfani, idan an rage tasirin amsawa, ana iya ƙara CALB yadda ya kamata kuma a ci gaba da amfani.Idan an rage tasirin amsawa da gaske, yana buƙatar maye gurbinsa.