Nucloside phosphoryalse (NP)

Takaitaccen Bayani:

Game da sinadarin nucleoside phosphoryalse

Akwai nau'ikan samfuran NP enzyme guda 3 (Lambar da aka haɗa da ES-NP-101~ ES-NP-103) waɗanda SyncoZymes suka ƙirƙira. ES-NP-101 purine nucleoside phosphorylase ne, ES-NP-102 da ES-NP-103 pyrimidine nucleoside phosphorylase ne. Nucleosidephosphorylase na iya raba nucleosides zuwa tushe da pentose phosphate. Ana iya raba nucleoside phosphorylase zuwa purine nucleoside phosphorylase da pyrimidine nucleoside phosphorylase bisa ga fifikon tushen nucleoside. Purine nucleoside phosphorylase na iya metabolize adenosine zuwa adenine, inosine zuwa hypoxanthine, guanosine zuwa guanine, kuma yana samar da ribose phosphate a lokaci guda. Pyrimidine nucleoside phosphorylase na iya metabolize uridine zuwa uracil kuma yana samar da ribose phosphate.

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Imel:lchen@syncozymes.com

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Alamun Samfura

Nau'in amsawar Nucloside phosphoryalse:

Bayanin Samfura:

Enzymes	Lambar Samfura	Ƙayyadewa
Foda na Enzyme	ES-NP-101~ ES-NP-103	saitin Nuclosidephosphoryalse guda 3, 50 MG kowanne 3 * 50mg / abu, ko wani adadi

Tsarin gwajin halayen Nucloside phosphoryalse:

Ana iya amfani da tsarin amsawa mai zuwa don tunani:
1-10 mg/ml Substrate
10 mg/ml enzyme
Ma'aunin Phosphate 100 mM (pH7.0)
A saka halayen a cikin na'urar girgiza zafin jiki akai-akai (misali, 30oC, 150rpm) na tsawon awanni 12-24. A naɗe kowace cakuda a cikin na'urar sanyaya iska don ƙara yawan furotin, ana iya amfani da sinadarin da ke cikinta don nazarin TLC, HPLC ko GC.