Phenylalanine ammonia lyase (PAL)
Takaitaccen Bayani:
Game da Phenylalanine ammonia lyase
ES-PAL: Wani nau'in enzymes wanda ke haifar da deamination kai tsaye na L-phenylalanine zuwa trans-cinnamic acid.Syncozymes sun haɓaka abubuwa 10 na phenylalanine ammonia lyase (mai lamba ES-PAL-101 ~ ES-PAL-110), waɗanda za a iya amfani da su a cikin deamination ko juyar da martani na phenylalanine da abubuwan da suka samo asali.
Nau'in amsawar catalytic:
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Imel:lchen@syncozymes.com
| Enzymes | Lambar samfur | Ƙayyadaddun bayanai |
| Enzyme Foda | ES-PAL-101~ ES-PAL-110 | wani sa na 10 L-phenylalanine ammonia-lyase, 50 MG kowane 10 abubuwa * 50mg / abu, ko wasu yawa |
★ High substrate takamaiman.
★ Karfin chiral selectivity.
★ Babban juzu'i.
★ Kadan kayan masarufi.
★ Sharuɗɗan ɗaukar hankali.
★ Abokan Muhalli.
➢ Yawanci, tsarin amsa ya kamata ya haɗa da substrate, maganin buffer da enzyme.
➢ Kowane nau'i na ES-PAL wanda ya dace da yanayi daban-daban mafi dacewa, wanda za'a iya yin nazari akai-akai.
➢ Ya kamata a ƙara ES-PAL na ƙarshe cikin tsarin amsawa don kula da ayyukan.
Misali 1 (Biosynthesis na trans cinnamic acid daga phenylalanine)(1):
Misali 2 (Biosynthesis na phenylalanine wanda aka samu daga trans-cinnamic acid wanda aka samu)(2):
Ci gaba da shekaru 2 ƙasa -20 ℃.
Kada a taɓa hulɗa da matsananciyar yanayi kamar: babban zafin jiki, high / low pH da babban taro kwayoyin kaushi.
1. Zachary Mays, Karishma Mohan,da al.Chem Commun (Camb).2020, 56, 5255-5258.
2. Ian Rowles, Bas Groenendaal,da al. Tetrahedron,2016. 72, 7343-7347.
