Phosphokinase (PKase)
Enzymes | Lambar samfur | Ƙayyadaddun bayanai |
Kit ɗin Nuna (SynKit) | ES-PKase-101~ES-PKase-121 | saitin 21 Ketoreductases, 1 MG kowane 21 abubuwa * 1 MG / abu |
★ High substrate takamaiman.
★ Karfin chiral selectivity.
★ Babban juzu'i.
★ Kadan kayan masarufi.
★ Sharuɗɗan ɗaukar hankali.
★ Abokan Muhalli.
➢ Yawanci, tsarin amsawa ya kamata ya haɗa da substrate, maganin buffer, enzyme, ATP, Mg2+.
➢ Ya kamata a ƙara PKase na ƙarshe cikin tsarin amsawa, bayan an daidaita pH da zafin jiki zuwa yanayin amsawa.
Misali 1 (Hadarin nicotinamide ribose phosphate daga nicotinamide ribose)(1):
Lura: misalan aikace-aikace da nassoshi an yi niyya ne don nuna iyakar aikace-aikacen PCase don sauƙin fahimta, kuma basu dace da takamaiman enzyme na SyncoZymes ba.
Ci gaba da shekaru 2 ƙasa -20 ℃.
Kada a taɓa hulɗa da matsananciyar yanayi kamar: babban zafin jiki, high / low pH da babban taro kwayoyin kaushi.
1. Khan, Javed A., Song Xiang, da Liang Tong.Tsarin 15.8 (2007): 1005-1013.