CALB mara motsi
Ana hana CALB motsi ta hanyar shaƙar jiki a kan resin mai tsananin hydrophobic wanda yake polymer mai yawan porous, styrene/methacrylate. CALB mai hana motsi ya dace da amfani a cikin sinadarai masu narkewa na halitta da tsarin da ba su da narkewa, kuma ana iya sake yin amfani da shi kuma a sake amfani da shi na tsawon lokaci a cikin yanayi mai dacewa.
Lambar samfur: SZ-CALB- IMMO100A, SZ-CALB- IMMO100B.
★Ayyuka masu yawa, zaɓin chiral mai yawa da kuma kwanciyar hankali mai yawa.
★Ingantaccen aiki a cikin matakan da ba na ruwa ba.
★Cire shi cikin sauƙi daga tsarin amsawa, da sauri dakatar da amsawa, da kuma guje wa ragowar furotin a cikin samfurin.
★Ana iya sake amfani da shi sannan a sake amfani da shi don rage farashin.
| Aiki | ≥10000PLU/g |
| Matsayin pH don amsawa | 5-9 |
| Yanayin zafin jiki don amsawa | 10-60℃ |
| Bayyanar | CALB-IMMO100-A: Rawaya mai haske zuwa launin ruwan kasa mai kauri CALB-IMMO100-B: Fari zuwa launin ruwan kasa mai haske mai kauri |
| Girman ƙwayoyin cuta | 300-500μm |
| Asarar bushewa a 105℃ | 0.5%-3.0% |
| Guduro don hana motsi | Babban porous, styrene/methacrylate polymer |
| Maganin narkewar amsawa | Ruwa, sinadarin narkewar halitta, da sauransu, ko kuma ba tare da sinadarin narkewar halitta ba. Don amsawar da ke cikin wasu sinadarai masu narkewar halitta, ana iya ƙara kashi 3% na ruwa don inganta tasirin amsawar. |
| Girman ƙwayoyin cuta | CALB-IMMO100-A: 200-800 μm CALB-IMMO100-B: 400-1200 μm |
Ma'anar raka'a: Raka'a 1 ta yi daidai da haɗakar propyl laurate 1μmol a minti ɗaya daga lauric acid da 1-propanol a 60℃. CALB-IMMP100-A da CALB-IMMO100-B da ke sama sun yi daidai da masu ɗaukar kaya marasa motsi waɗanda ke da girman ƙwayoyin cuta daban-daban.
1. Nau'in reactor
Enzyme ɗin da aka hana motsi yana aiki ga na'urar sarrafa kettle batch da kuma na'urar sarrafa kwararar ruwa mai ci gaba da kwanciya. Ya kamata a lura cewa ya kamata a guji niƙawa saboda ƙarfin waje yayin ciyarwa ko cikawa.
2. pH na amsawa, zafin jiki da kuma sinadarin narkewa
Ya kamata a ƙara enzyme ɗin da aka hana motsi a ƙarshe, bayan an ƙara wasu kayan kuma an narkar da su, sannan a daidaita pH.
Idan amfani da substrate ko samuwar samfurin zai haifar da canjin pH yayin amsawar, ya kamata a ƙara isasshen ma'aunin zafi a cikin tsarin amsawar, ko kuma a sa ido kan pH ɗin kuma a daidaita shi yayin amsawar.
A cikin kewayon jure yanayin zafi na CALB (ƙasa da 60 ℃), saurin juyawa ya ƙaru tare da ƙaruwar zafin jiki. A aikace, ya kamata a zaɓi zafin amsawa gwargwadon kwanciyar hankali na substrate ko samfurin.
Gabaɗaya, amsawar hydrolysis na ester ya dace a tsarin lokaci na ruwa, yayin da amsawar haɗin ester ya dace a tsarin lokaci na organic. Maganin narkewar halitta na iya zama ethanol, tetrahydrofuran, n-hexane, n-heptane da toluene, ko kuma maganin narkewar da ya dace. Don amsawar a cikin wasu sinadarai na organic, ana iya ƙara kashi 3% na ruwa don inganta tasirin amsawar.
3. Sake amfani da kuma tsawon lokacin sabis na CALB
A ƙarƙashin yanayin amsawar da ya dace, ana iya dawo da CALB kuma a sake amfani da shi, kuma takamaiman lokutan aikace-aikacen sun bambanta dangane da ayyuka daban-daban.
Idan ba a sake amfani da CALB da aka dawo da shi akai-akai ba kuma yana buƙatar a adana shi bayan an warke, yana buƙatar a wanke shi a busar da shi sannan a rufe shi a zafin 2-8 ℃.
Bayan an yi amfani da shi sau da yawa, idan an rage ƙarfin amsawar, ana iya ƙara CALB yadda ya kamata kuma a ci gaba da amfani da shi. Idan ƙarfin amsawar ya ragu sosai, yana buƙatar a maye gurbinsa.
Misali na 1 (Aminolysis)(1):
Misali na 2 (Aminolysis)(2):
Misali na 3 (Haɗarin polyester mai buɗe zobe)(3):
Misali na 4 (Transesterification, regioselective na rukunin hydroxyl)(4):
Misali na 5 (Transesterification, ƙudurin motsi na barasa mai launin fata)(5):
Misali na 6(Esterification, ƙudurin motsi na carboxylic acid)(6):
Misali na 7 (Esterolysis, ƙudurin motsi)(7):
Misali na 8 (Hydrolysis na amides)(8):
Misali na 9 (Acylation of amines)(9):
Misali na 10 (Abin da ya ƙara Aza-Michael)(10):
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7. Souza TC, Fonseca TS, Costa JA, da tal. J. Mol. Katal. B: Enzyme, 2016, 130: 58-69.
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9. Joubioux FL, Henda YB, Bridiau N, da tal. J. Mol. Katal. B: Enzyme, 2013, 85-86: 193-199.
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