Nitrile hydratase (NHT)
Takaitaccen Bayani:
Game da ES-NHTs
ES-NHTs: wani nau'in metalloenzyme ne, wanda ke sanya nitriles cikin sinadarin amide mai kama da juna. Ana amfani da su sosai ba wai kawai don haɗa amino acid, amides, carboxylic acid da abubuwan da suka samo asali ba, har ma don shirya magungunan chiral. Akwai nau'ikan nitrile hydratases guda 24 da SyncoZymes ya samar (Lambar da aka samar a matsayin ES-NHT-101～ES-NHT-124).
Nau'in amsawar catalytic:
Wayar Salula/Wechat/WhatsApp: +86-13681683526
Imel:lchen@syncozymes.com
| Enzymes | Lambar Samfura | Ƙayyadewa |
| Foda na Enzyme | ES-NHT-101~ ES-NHT-124 | saitin Nitrile Hydratases 24, 50 MG kowanne 24 * 50mg / abu, ko wani adadi |
| Kayan tantancewa (SynKit) | ES-NHT-2400 | saitin Nnitrile Hydratases 24, 1 mg kowanne 24 * 1mg / abu |
★ Babban takamaiman substrate.
★ Ƙarfin zaɓin chiral.
★ Babban canji.
★ Rage yawan kayayyakin da aka samar.
★ Yanayin amsawa mai sauƙi.
★ Mai kyau ga muhalli.
➢ A al'ada, tsarin amsawar yakamata ya haɗa da substrate, maganin buffer (mafi kyawun amsawar pH) da ES-NHT.
➢ Ana iya gwada duk ES-NHTs bi da bi a cikin tsarin amsawar da ke sama ko tare da Kayan Binciken NHT (SynKit NHT).
➢ Ya kamata a yi nazarin dukkan nau'ikan ES-NHTs da suka dace da yanayin amsawar da ya dace daban-daban.
➢ Yawan sinadarin substrate ko samfurin da ke ɗauke da shi na iya hana ayyukan ES-NHT. Duk da haka, ana iya rage wannan hanawa ta hanyar ƙara substrate a cikin rukuni, haɗawa da amidases ko cire samfurin.
Misali na 1 (haɗin kai da amidase)(1):
Misali na 2(2):
Ajiye shi a wuri mai sanyi na tsawon shekaru 2 ƙasa da -20 ℃.
Kada a taɓa yin hulɗa da yanayi mai tsanani kamar: zafin jiki mai yawa, pH mai yawa/ƙaranci da kuma yawan sinadarin sinadarai masu narkewa.
1 Vojtech V, Ludmila M, Alicja BV, da tal. J Mol Catal B-enzym, 2011, 71: 51-55.
2 Gary W. B, Thomas G, Christopher B. M, da sauransu. Tetrahedron lett, 2010, 51: 1639-1641.
