Nitrile reductase (NRED)
Takaitaccen Bayani:
Game da Nitrile Reductase
Akwai nau'ikan samfurin enzyme na NRED guda 3 (Lambar kamar ES-NRED101~ ES-NRED103) wanda SyncoZymes ya ƙirƙira. NRED na iya haɓaka rage rukunin nitrile zuwa amine. A cikin tsarin catalysis, ana buƙatar NADPH a matsayin mai jigilar hydrogen.
Nau'in amsawar nitrile reductasecatalytic
Wayar Salula/Wechat/WhatsApp: +86-13681683526
Imel:lchen@syncozymes.com
| Enzymes | Lambar Samfura | Ƙayyadewa |
| Ƙayyadewa | ES- NRED -101~ ES- NRED -103 | saitin Nitrile Reductase guda 3, 50 MG kowanne 3 * 50mg / abu, ko wani adadi |
Ana iya amfani da tsarin amsawa mai zuwa don tunani:
1-10 mg/ml Substrate
10 mg/ml enzyme
10% (v/v) Ruwan da ke ɗauke da sinadarai masu guba (1)
Ma'aunin Phosphate 100 mM (pH7.0)
0.2 mg/ml NADP+
10 mg/ml Glucose
2 mg/ml Glucose dehydrogenase (GDH)
A saka halayen a cikin na'urar girgiza zafin jiki akai-akai (misali, 30oC, 150rpm) na tsawon awanni 24 ~ 48. A sanya na'urar sanyaya iska a kowane cakuda don ƙara yawan furotin, ana iya amfani da su wajen nazarin TLC, HPLC ko GC.
Lura (1): Maganin narkewar halitta (kamar DMSO, methanol, acetonitrile, IPA, da sauransu) zaɓi ne, ana amfani da shi don abubuwan da ba sa narkewa.
